LDLR Recombinant Protein

Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.

Specyfikacja/Zawartość:

• Tested Applications: WB
• Applications: This recombinant protein can be used for WB. For research use only.
• Predicted Molecular Weight: 86 kDa
• Physical state: Lyophilized
• Buffer: PBS, pH7.4
• Concentration: N/A
• NCBI official symbol: LDLR
• Accession #: NP_000518.1
• Protein GI: N/A
• NCBI gene ID#: 3949
• NCBI official full name: low density lipoprotein receptor
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: P01130
• Background Reference 1: Yamamoto T, et al., 1984, Cell 39 : 27–38.
• Background Reference 2: Nykjaer A, Willnow TE, 2002, Trends Cell Biol. 12 (6): 273–80.
• Background Reference 3: Barrett P.H., Watts G.F. 2002, Atherosclerosis. Supplements. 2: 1-4.
• Background Reference 4: Nomura S. et al., 2004, Gene. Therapy. 11: 1540-1548.
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: His Tag
• Sequence: Ala 22 - Arg 788
• Biology activity: (1) Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag at 10 ug/mL (100 ul/well) can bind Biotinylated Human PCSK9 with a linear range of 0.01-0.1 ug/mL. (2) Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag at 10 ug/mL (100 ul/well) can bind Biotinylated Human (D374Y) PCSK9 with a linear range of 0.02-0.5 ug/mL.
• Purity: >90% as determined by SDS-PAGE.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.