CD47 Recombinant Protein

Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Specyfikacja/Zawartość:

• Tested Applications: WB
• Applications: This recombinant protein can be used for WB. For research use only.
• Predicted Molecular Weight: 40.4 kDa
• Physical state: Lyophilized
• Buffer: 50 mM tris, 100 mM glycine, pH7.5
• Concentration: N/A
• NCBI official symbol: CD47
• Accession #: NP_942088
• Protein GI: N/A
• NCBI gene ID#: 961
• NCBI official full name: CD47 molecule
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: Q08722
• Background Reference 1: Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.
• Background Reference 2: Latour S., et al., 2001, J. Immunol. 167:2547-2554.
• Background Reference 3: Piccio L., et al., 2005, Blood 105:2421-2427.
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: C-Fc Tag
• Sequence: Gln 19 - Pro 139
• Biology activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47, Fc Tag at 2 µg/ml can bind Human SIRP alpha, His Tag with a linear range of 16-250 ng/mL.
• Purity: >95% as determined by SDS-PAGE and more than 90% measured by SEC-HPLC.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.