Complement C5 Recombinant Protein

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

Specyfikacja/Zawartość:

• Tested Applications: E, WB
• Applications: This protein carries a polyhistidine tag at the C-terminus.The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.9 kDa (α
• Predicted Molecular Weight: 73.9 kDa (β chain) & 114.9 kDa (α chain)
• Physical state: Lyophilized
• Buffer: PBS, pH7.4
• Concentration: N/A
• NCBI official symbol: C5
• Accession #: XP_005580972.1
• Protein GI: N/A
• NCBI gene ID#: 102125658
• NCBI official full name: complement C5
• NCBI organism: Macaca fascicularis
• Peptide sequence: N/A
• SWISSPROT #: N/A
• Background Reference 1: DeMartino JA, et al. 1994. J Biol Chem. 269(20):14446-50.
• Background Reference 2: Fredslund F, et al. 2008. Nat Immunol. 9(7):753-60.
• Background Reference 3: Chen Z, et al. 1998. J Biol Chem. 273(17):10411-9.
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Cynomolgus monkey
• By Source: Human Cells
• By Species: Other
• Fusion tag: His Tag
• Sequence: Gln 19 - Cys 1676
• Biology activity: N/A
• Purity: >95% as determined by SDS-PAGE.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.