Complement Component C5a Recombinant Protein

Complement Component C5a (C5a) is also known as C5,and is a protein fragment released from complement component C5.,is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

Specyfikacja/Zawartość:

• Tested Applications: E, WB
• Applications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Predicted Molecular Weight: 34.6 kDa
• Physical state: Lyophilized
• Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
• Concentration: N/A
• NCBI official symbol: C5
• Accession #: XP_015292262.1
• Protein GI: N/A
• NCBI gene ID#: 102125658
• NCBI official full name: complementC5
• NCBI organism: Macaca fascicularis
• Peptide sequence: N/A
• SWISSPROT #: N/A
• Background Reference 1: Gerard, C. and N.P. Gerard, 1994, Annu. Rev. Immunol.12:775.
• Background Reference 2: Monk, PN. et al., 2007, British Journal of Pharmacology. 152: 429-48.
• Background Reference 3: Reis ES, et al., 2011,Brain Behav Immun. Apr 23.
• Background Reference 4: Bergseth G,et al., 2011, Ann Thorac Surg., 91(2):527-33.
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: Fc Tag
• Sequence: Leu 679 - Arg 751
• Biology activity: N/A
• Purity: >90% as determined by SDS-PAGE.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.