CD40 Recombinant Protein

CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

Specyfikacja/Zawartość:

• Tested Applications: E, WB
• Applications: This recombinant protein can be used for E, WB. For research use only.
• Predicted Molecular Weight: 45.9 kDa
• Physical state: Lyophilized
• Buffer: 50 mM Tris, 100 mM Glycine, pH7.5
• Concentration: N/A
• NCBI official symbol: CD40
• Accession #: NP_001252791.1
• Protein GI: N/A
• NCBI gene ID#: 707749
• NCBI official full name: tumor necrosis factor receptor superfamily member 5 precursor
• NCBI organism: Macaca mulatta
• Peptide sequence: Glu 21 - Arg 193
• SWISSPROT #: NP_001252791.1
• Background Reference 1: Banchereau, J. et al., 1994, Annu. Rev. Immunol. 12: 881-922.
• Background Reference 2: Ni, C.Z. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 10395-10399.
• Background Reference 3: Ferrari.S. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 12614-12619.
• Background Reference 4: Bhushan, A and Covey, L.R. 2001, Immunol. Res. 24: 311-324.
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Rhesus monkey
• By Source: Human Cells
• By Species: Other
• Fusion tag: Fc Tag
• Sequence: Glu 21 - Arg 193
• Biology activity: Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque CD40, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD40 Ligand, His Tag with a linear range of 5-50 ng/mL.
• Purity: >95% as determined by SDS-PAGE.
• Endotoxin level is less than 1.0 EU per ug by the LAL method.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.