CD3D Recombinant Protein

CD3D is also known as CD3-DELTA, T3D, and is a single-pass type I membrane protein. CD3D is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.

Specyfikacja/Zawartość:

• Tested Applications: WB
• Applications: This recombinant protein can be used for WB. For research use only.
• Predicted Molecular Weight: 11.4 kDa
• Physical state: Lyophilized
• Buffer: PBS, pH7.4
• Concentration: N/A
• NCBI official symbol: CD3D
• Accession #: NP_000723.1
• Protein GI: N/A
• NCBI gene ID#: 915
• NCBI official full name: CD3d molecule, delta (CD3-TCR complex)
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: P04234
• Background Reference 1: Gil J, et al., 2011, J Clin Invest, 121(10):3872-6.
• Background Reference 2: Lu X, et al.,2011, Hematology, 16(3):185-9.
• Background Reference 3: Kim ST, et al., 2010, J Immunol, 185(5):2951-9.
• Background Reference 4: Holst, J. et al., 2008, Nat Immunol, 9 (6): 658-66.
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: His Tag
• Sequence: Phe 22 - Ala 105
• Biology activity: N/A
• Purity: >90% as determined by SDS-PAGE.
• Endotoxin level is less than 1.0 EU per ug by the LAL method.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.