CD27 Recombinant Protein

CD27 (TNFRSF7) is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, mediated by the adaptor proteins TRAF2 and TRAF5. In addition, the proapoptotic protein SIVA is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

Specyfikacja/Zawartość:

• Tested Applications: N/A
• Applications: This recombinant proteins is for research use only.
• Predicted Molecular Weight: N/A
• Physical state: Lyophilized
• Buffer: Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.
• Concentration: N/A
• NCBI official symbol: CD27
• Accession #: AAH12160
• Protein GI: 15082491
• NCBI gene ID#: 939
• NCBI official full name: CD27 molecule
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: Q9JHJ8
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: CHO cells
• Species: Human
• By Source: CHO Cells
• By Species: Human
• Fusion tag: Fc Tag
• Sequence: The extracellular domain of human CD27 (aa 21-192) is fused to the N-terminus of the Fc region of human IgG1.
• Biology activity: Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 ligand and anti-CD3.
• Purity: ≥98% (SDS-PAGE).
• Endotoxin level is less than 0.06EU/ µg protein (LAL test
• Lonza).

Wysyłka i przechowywanie:

Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.