Complement Component C2 Recombinant Protein

C2 is a major histocompatibility complex class-III protein. Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. The lectin (LP) and classical (CP) pathways are two of the three main activation cascades of the complement system. These pathways start with recognition of different pathogen- or danger-associated molecular patterns and include identical steps of proteolytic activation of complement component C4, formation of the C3 proconvertase C4b2, followed by cleavage of complement component C2 within C4b2 resulting in the C3 convertase C4b2a.

Specyfikacja/Zawartość:

• Tested Applications: WB
• Applications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
• Predicted Molecular Weight: 83.0 kDa
• Physical state: Lyophilized
• Buffer: 20 mM Tris, 150 mM NaCl, pH7.5
• Concentration: N/A
• NCBI official symbol: C2
• Accession #: NP_000054.2
• Protein GI: N/A
• NCBI gene ID#: 717
• NCBI official full name: complementC2
• NCBI organism: Homo sapiens
• Peptide sequence: N/A
• SWISSPROT #: P06681
• Background Reference 1: Mortensen S, et al. 2016. J Biol Chem. 291(32):16494-507.
• Background Reference 2: Yaseen S, et al. 2017. FASEB J. 31(5):2210-2219.
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human
• By Source: Human Cells
• By Species: Human
• Fusion tag: His Tag
• Sequence: Ala 21 - Leu 752
• Biology activity: N/A
• Purity: >90% as determined by SDS-PAGE.

Wysyłka i przechowywanie:

Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.