APRIL multimeric Recombinant Protein

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Specyfikacja/Zawartość:

• Tested Applications: N/A
• Applications: This recombinant proteins is for research use only.
• Predicted Molecular Weight: N/A
• Physical state: Lyophilized
• Buffer: Contains PBS + 0.5% Trehalose. Reconstitute with 100 µl sterile water.
• Concentration: 0.1mg/ml after reconstitution.
• NCBI official symbol: Tnfsf13
• Accession #: Q9D777
• Protein GI: 21363035
• NCBI gene ID#: 69583
• NCBI official full name: tumor necrosis factor (ligand) superfamily, member 13
• NCBI organism: Mus musculus
• Peptide sequence: N/A
• SWISSPROT #: Q8R459
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: Human, Mouse
• By Source: Human Cells
• By Species: Human, Mouse
• Fusion tag: DDDDK Tag
• Sequence: The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-110) and a DDDDK-tag.
• Biology activity: Stimulates B cell proliferation.
• Purity: >95% (SDS-PAGE).
• Endotoxin level is less than 0.02EU/ µg purified protein (LAL test
• Lonza).

Wysyłka i przechowywanie:

Stable for at least 6 months after receipt when stored at -20°C.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.