Staphylococcus Glu-C Recombinant Protein

Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Glu-C cleaves at the Carboxyl side of E (can also cleave D under certain conditions). Recombinant Staphylococcus Glu-C is a 28.8 kDa protease consisting of 266 amino acid residues.

Specyfikacja/Zawartość:

• Gene name: Glu-C
• Host: E. coli
• Label: None
• Applications: Cleaves at the Carboxyl side of E (can also cleave D under certain conditions).
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Endotoxin levels: < 0.1 ng/µg of protein (<1EU/µg)
• Reconstitution Buffer: water
• Buffer: 10mMTris, pH 8.0
• Additives/Stabilizer: None
• Form: lyophilized
• Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
• Reconstitution tips: Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at ‑20°C to -80°C.
• MW: 28.8 kDa
• Length (aa): 266
• Protein Sequence: LPNNDRHQIT DTTNGHYAPV TYIQVEAPTG TFIASGVVVG KDTLLTNKHV VDATHGDPHA LKAFPSAINQ DNYPNGGFTA EQITKYSGEG DLAIVKFSPN EQNKHIGEVV KPATMSNNAE TQVNQNITVT GYPGDKPVAT MWESKGKITY LKGEAMQYDL STTGGNSGSP VFNEKNEVIG IHWGGVPNEF NGAVFINENV RNFLKQNIED IHFANDDQPN NPDNPDNPNN PDNPNNPDEP NNPDNPNNPD NPDNGDNNNS DNPDAA
• NCBI GeneID: 28381405
• Accession Number Protein: WP_000676548.1
• Accession Numberm RNA: NZ_MAQP01000013.1.
• Uniprot: P0C1U8

Inne:

For research use only.