Autophagy Protein Detection Set

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. PIST is a protein that interacts with Beclin-1 through its coiled-coil domain and can modulate Beclin-1 activity. LAMP1 and LAMP2 are homologous, highly glycosylated proteins associated with the lysosome and are thought to have overlapping functions. Mice lacking LAMP1 have very minor defects compared to those deficient in LAMP2 expression. However, the loss of both proteins results in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.;;For images please see PDF data sheet

Dodatkowe informacje:

Optimal dilutions for each application to be determined by the researcher.

Specyfikacja/Zawartość:

• Host Species: N/A
• Species Reactivity: Human
• Homology: N/A
• Immunogen: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
• Tested Applications: WB, IHC, IF
• Applications: N/A
• Specificity: N/A
• Purification: Antibodies are supplied as affinity chromatography purified IgG.
• Clonality: Polyclonal
• Isotype: N/A
• Conjugate: N/A
• Physical State: Liquid
• Buffer: PBS containing 0.02% sodium azide.
• Concentration: Antibody 1 mg/mL
• Set Contents: APG7 Antibody (CT), Catalog No. 3615 (50 µg). Beclin-1 Antibody (CT), Catalog No. 3611 (50 µg). LAMP1 Antibody, Catalog No. 3629 (50 µg). LAMP2 Antibody, Catalog No. 3627 (50 µg). PIST Antibody (CT), Catalog No. 3631 (50 µg)
• Products: 3615, 3611, 3629, 3627, 3631

Wysyłka i przechowywanie:

• Stable at 4°C for three months, store at -20°C for up to one year.
• Shipping Condition: Not Specified

Inne:

For Research Use Only