HMGB1 Antibody
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The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).
Dodatkowe informacje:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Specyfikacja/Zawartość:
- Research area: Innate Immunity
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Homology: Predicted species reactivity based on immunogen sequence: Chicken: (100%), Pig: (100%)
- Immunogen: Anti-HMGB1 antibody (7715) was raised against a peptide corresponding to 19 amino acids near the center of human HMGB1.
- The immunogen is located within 70-120 amino acids of HMGB1.
- Tested Applications: E, WB, IHC-P, IF
- Applications: WB: 1 μ
- g/mL
- IHC: 1-2 μ
- g/mL
- IF: 20 μ
- g/mL.
- Antibody validated: Western Blot in human, mouse and rat samples
- Immunohistochemistry in human, mouse, and rat samples
- Immunofluorescence in human samples. All other applications and species not yet tested.
- Specificity: HMGB1 antibody is human, mouse and rat reactive.
- Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
- Positive control 2: Cat. No. 10-101 - Human Lung Tissue Slide
- Positive control 3: N/A
- Positive control 4: N/A
- Positive control 5: N/A
- Positive control 6: N/A
- Predicted molecular weight: Predicted: 25 kDa
- Observed: 25 kDa
- MW explanation: N/A
- Validation:
KO Validation (Figure 1): Anti-HMGB1 antibodies (7715) specificity was further verified by HMGB1 specific knockout cell lysate. HMGB1 signal was detected in wild type cells, but not in the HMGB1 knockout cells.
. - Isoforms: Human HMGB1 has only 1 isoform (215aa, 25kD). Mouse HMGB1 has only 1 isoform (215aa, 25kD). Rat HMGB1 has only 1 isoform (215aa, 25kD). This antibody detects HMGB1 of human, mouse and rat.
- Purification: HMGB1 antibody is affinity chromatography purified via peptide column.
- Clonality: Polyclonal
- Clone: N/A
- Isotype: IgG
- Conjugate: Unconjugated
- Physical state: Liquid
- Buffer: HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.
- Concentration: 1 mg/mL
- NCBI official symbol: HMGB1
- Accession #: NP_002119
- Protein GI #: 4504425
- NCBI gene ID#: 3146
- NCBI official full name: high mobility group box 1
- NCBI organism: homo sapiens
- SWISSPROT #: P09429
Wysyłka i przechowywanie:
- HMGB1 antibody can be stored at 4˚
- C for three months and -20˚
- C, stable for up to one year.
Inne:
Optimal dilutions for each application to be determined by the researcher.
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