IL-12A Antibody

IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1). IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2). The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2). Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Immunology, Innate Immunity
• Host species: Rabbit
• Species reactivity: Human
• Homology: Predicted species reactivity based on immunogen sequence: Llama: (94%), Goat: (89%), Pig: (89%), Sheep: (89%), Horse: (83%), Bovine: (83%)
• Immunogen: IL-12A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IL-12A.
• The immunogen is located within the last 50 amino acids of IL-12A.
• Tested Applications: E, WB, IHC-P, IF
• Applications: IL-12A antibody can be used for detection of IL-12A by Western blot at 0.5 - 1 μ
• g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: IL-12A antibody is human specific. IL-12A antibody will not cross-react with IL-12B.
• Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 22 kDa
• Observed: 22 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: IL-12A antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: IL-12A antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: IL 12A
• Accession #: NP_000873
• Protein GI #: 24430219
• NCBI gene ID#: 3592
• NCBI official full name: interleukin 12A
• NCBI organism: homo sapiens
• SWISSPROT #: P29459

Wysyłka i przechowywanie:

• IL-12A antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year.

Inne:

Optimal dilutions for each application to be determined by the researcher.