ANG Antibody

ANG Antibody: Angiogenin (ANG or ANG I) is important for the process of neovascularization and formation of new blood vessels. ANG is similar to pancreatic ribonuclease A and functions as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. It interacts with endothelial cell-surface actin and may cause changes in the cell cytoskeleton. ANG is thought to be involved in the development of solid tumors and its antagonists are capable of inhibiting tumor growth. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). Angiogenin is a genetic link between ALS and PD.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Cancer, Cell Cycle
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Horse: (75%)
• Immunogen: ANG antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG.
• The immunogen is located within amino acids 70 - 120 of ANG.
• Tested Applications: E, WB, IF
• Applications: ANG antibody can be used for detection of ANG by Western blot at 1 - 2 μ
• g/mL. For immunofluorescence start at 20 μ
• g/mL.
• Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Specificity: Two alternatively spliced transcript variants have been observed.
• Positive control 1: Cat. No. 1464 - Rat Liver Tissue Lysate
• Positive control 2: Cat. No. 10-201 - Human Liver Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: 16 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: ANG Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: ANG Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: ANG
• Accession #: NP_001136
• Protein GI #: 4557313
• NCBI gene ID#: 283
• NCBI official full name: angiogenin, ribonuclease, RNase A family, 5
• NCBI organism: homo sapiens
• SWISSPROT #: P03950

Wysyłka i przechowywanie:

• ANG antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.