PPAPDC1B Antibody

PPAPDC1B Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Signal Transduction
• Host species: Rabbit
• Species reactivity: Human, Mouse
• Homology: N/A
• Immunogen: PPAPDC1B antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human PPAPDC1B.
• The immunogen is located within amino acids 30 - 80 of PPAPDC1B.
• Tested Applications: E, WB
• Applications: PPAPDC1B antibody can be used for detection of PPAPDC1B by Western blot at 1 - 2 μ
• g/mL.
• Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1287 - EL4 Cell Lysate
• Positive control 2: N/A
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: N/A
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: PPAPDC1B Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: PPAPDC1B Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: PPAPDC1B
• Accession #: NP_001096029
• Protein GI #: 156523237
• NCBI gene ID#: 84513
• NCBI official full name: phosphatidic acid phosphatase type 2 domain containing 1B
• NCBI organism: Homo sapiens
• SWISSPROT #: Q8NEB5

Wysyłka i przechowywanie:

• PPAPDC1B antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.