ACE2 Antibody

ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Infectious Disease
• Host species: Rabbit
• Species reactivity: Human, Mouse, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Bovine: (93%)
• Immunogen: ACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ACE2.
• The immunogen is located within amino acids 150 - 200 of ACE2.
• Tested Applications: E, WB, IHC-P, IF
• Applications: WB: 4 μ
• g/mL
• IHC: 2 μ
• g/mL
• IF: 20 μ
• g/mL.
• Antibody validated: Western Blot in human, mouse and rat samples
• Immunohistochemistry in human, mouse and rat samples
• Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Specificity: Anti-ACE2 has no cross response to ACE1.
• Positive control 1: Cat. No. 1305 - Human Kidney Tissue Lysate
• Positive control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 93kD
• Observed: 130 kD (7 N-linked glycosylation)
• MW explanation: N/A
• Validation: N/A
• Isoforms: Human ACE2 has 2 isoforms, including isoform 1 (805aa, 93kD) and isoform 2 (555aa, 64kD). Mouse ACE2 also has 2 isoforms, including isoform 1 (805aa, 92kD) and isoform 2 (353aa, 40kD). Rat ACE2 has one isoform (805aa, 93kD). 3229 can detect human, mouse and rat.
• Purification: ACE2 Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: ACE2
• Accession #: NP_068576
• Protein GI #: 11225609
• NCBI gene ID#: 59272
• NCBI official full name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• NCBI organism: Homo sapiens
• SWISSPROT #: Q9BYF1

Wysyłka i przechowywanie:

• ACE2 antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.