IL-22 Receptor Antibody

IL-22 Receptor Antibody: A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.

Dodatkowe informacje:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Specyfikacja/Zawartość:

• Research area: Chemokines & Cytokines
• Host species: Rabbit
• Species reactivity: Human, Rat
• Homology: Predicted species reactivity based on immunogen sequence: Mouse: (93%), Bovine: (88%)
• Immunogen: IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor.
• The immunogen is located within the first 50 amino acids of IL-22 Receptor.
• Tested Applications: E, WB, IHC-P, ICC, IF
• Applications: IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5-1 μ
• g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
• g/mL and immunohistochemistry starting at 5 μ
• g/mL. For immunofluorescence start at 10 μ
• g/mL.               
• Antibody validated: Western Blot in human samples
• Immunohistochemistry in rat samples
• Immunocytochemistry in human samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Specificity: N/A
• Positive control 1: Cat. No. 1211 - HepG2 Cell Lysate
• Positive control 2: Cat. No. 17-011 - Hep G2 Cell Slide
• Positive control 3: N/A
• Positive control 4: N/A
• Positive control 5: N/A
• Positive control 6: N/A
• Predicted molecular weight: Predicted: 63 kDa
• Observed: 65 kDa
• MW explanation: N/A
• Validation: N/A
• Isoforms: N/A
• Purification: IL-22 Receptor Antibody is affinity chromatography purified via peptide column.
• Clonality: Polyclonal
• Clone: N/A
• Isotype: IgG
• Conjugate: Unconjugated
• Physical state: Liquid
• Buffer: IL-22 Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• NCBI official symbol: IL22RA1
• Accession #: NP_067081
• Protein GI #: 10719607
• NCBI gene ID#: 58985
• NCBI official full name: interleukin 22 receptor, alpha 1
• NCBI organism: Homo sapiens
• SWISSPROT #: Q8N6P7

Wysyłka i przechowywanie:

• IL-22 Receptor antibody can be stored at 4˚
• C for three months and -20˚
• C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Inne:

Optimal dilutions for each application to be determined by the researcher.