SARS CoV-2 full length spike protein in LMNG detergent

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor. The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways: MSP nanodiscs, based on MSP proteins Detergent Mycelles, as you can see here Synthetic nanodiscs

Specyfikacja/Zawartość:

• Tested Applications: E
• Applications: This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
• Predicted Molecular Weight: 1286 amino acids (including Rho1D4 tag and linker)
• 142114 Da
• Physical state: Liquid
• Buffer: 20 mM Hepes pH 7.5
• 150 mM NaCl, 0.01 % LMNG
• Concentration: batch dependent
• NCBI official symbol: S
• Accession #: N/A
• Protein GI: N/A
• NCBI gene ID#: 43740568
• NCBI official full name: surface glycoprotein
• NCBI organism: Severe acute respiratory syndrome coronavirus 2
• Peptide sequence: Full-length sequence (aa 1 – 1273),
• furin cleavage site “RRAR” mutated to “GSAG”
• C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
• SWISSPROT #: P0DTC2
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: HEK293 cells
• Species: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein
• By Source: Human Cells
• By Species: Other
• Fusion tag: C-terminal Rho1D4
• Sequence: Full-length sequence (aa 1 – 1273),
• furin cleavage site “RRAR” mutated to “GSAG”
• C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
• Biology activity: not determined
• Purity: >98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose.

Wysyłka i przechowywanie:

–80°C. Avoid freeze-thaw cycles

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.