Flagellin Recombinant Protein

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

Specyfikacja/Zawartość:

• Tested Applications: N/A
• Applications: This recombinant proteins is for research use only.
• Predicted Molecular Weight: N/A
• Physical state: Lyophilized
• Buffer: Contains PBS. Reconstitute with 100 µl sterile water.
• Concentration: 0.1mg/ml after reconstitution.
• NCBI official symbol: fliC
• Accession #: P06179
• Protein GI: 20141325
• NCBI gene ID#: 1253480
• NCBI official full name: flagellin
• NCBI organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
• Peptide sequence: N/A
• SWISSPROT #: P80370
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: E. coli
• Species: Human, Mouse
• By Source: E. Coli
• By Species: Human, Mouse
• Fusion tag: His Tag
• Sequence: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.
• Biology activity: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5 ug/ml on bone-marrow macrophages in the presence of streptolysin O (SLO), a protein that allows delivery of exogenous molecules into the cytosol of living cells.
• Purity: >95% (SDS-PAGE).
• Endotoxin level is less than 0.01EU/ µg purified protein (LAL test
• Lonza).

Wysyłka i przechowywanie:

Stable for at least 6 months after receipt when stored at -20°C.

Inne:

Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.