G-CSF Recombinant Protein

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant murine G-CSF is a 19.0 kDa protein consisting of 179 amino acid residues.

Specyfikacja/Zawartość:

• Tested Applications: N/A
• Applications: N/A
• Predicted Molecular Weight: N/A
• Physical state: Lyophilized
• Buffer: N/A
• Concentration: N/A
• NCBI official symbol: Csf3
• Accession #: NP_034101.1
• Protein GI: 6753536
• NCBI gene ID#: 12985
• NCBI official full name: colony stimulating factor 3 (granulocyte)
• NCBI organism: Mus musculus
• Peptide sequence: MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA
• SWISSPROT #: P09920
• Background Reference 1: N/A
• Background Reference 2: N/A
• Background Reference 3: N/A
• Background Reference 4: N/A
• Background Reference 5: N/A
• Source: E. coli
• Species: Murine
• By Source: E. Coli
• By Species: Mouse
• Fusion tag: N/A
• Sequence: MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA
• Biology activity: The ED50 was determined by the dose - dependent stimulation of the proliferation of murine M - NFS - 60 cells is < 0.05 ng/mL, corresponding to a specific activity of > 2 x 10⁷ units/mg.
• Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.
• Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Wysyłka i przechowywanie:

The lyophilized G-CSF recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted G-CSF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing G-CSF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Inne:

This product is for research use only.