Human Granzyme B ELISA kit

ELISA kit for the detection of Granzyme B in the research laboratory

Specyfikacja/Zawartość:

• Category: Research Kits
• Research Area: Proteases, Inhibitors, & Enzymes
• Specificity: NA
• Form & Buffer: NA
• Applications: ELISA
• Usage Recommendations: Optimal conditions to be determined by end user
• Assay Information: An antihuman Granzyme B coating antibody is adsorbed onto microwells. Human Granzyme B present in the sample or  standard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step and a biotinconjugated antihuman Granzyme B antibody is added and binds to  Granzyme B captured by the first antibody. Following incubation unbound biotin conjugated antihuman Granzyme B antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated  antihuman Granzyme B antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and  substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Granzyme B present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Granzyme B standard dilutions and human Granzyme B sample concentration determined.

Wysyłka i przechowywanie:

• Storage: Store at 2-8°C
• Shipping: Blue Ice