A competitive Inhibition ELISA kit for detection of Abscisic Acid from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
A monoclonal antibody specific to Abscisic Acid (ABA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Abscisic Acid (ABA) and unlabeled Abscisic Acid (ABA) (Standards or samples) with the pre-coated antibody specific to Abscisic Acid (ABA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Abscisic Acid (ABA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Abscisic Acid (ABA) in the sample.
- Species reactivity: General
- Sensitivity: 0.54ng/mL
- Detection range: 1.23-100ng/mL
- Standard solution concentration: 100ng/mL
- ELISA type: Competitive Inhibition
- Assay length: 2h
Wysyłka i przechowywanie:
The General ABA ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
This kit is intended for research use only. Do not use for diagnostics purposes.