ELISA kit for Rat LDHB (Lactate Dehydrogenase B)

A sandwich ELISA kit for detection of Lactate Dehydrogenase B from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Dodatkowe informacje:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase B (LDHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase B (LDHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase B (LDHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
• 10nm. The concentration of Lactate Dehydrogenase B (LDHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Specyfikacja/Zawartość:

• Species reactivity: Rat
• Sensitivity: 0.122ng/mL
• Detection range: 0.312-20ng/mL
• Standard solution concentration: 20ng/mL
• ELISA type: Sandwich
• Assay length: 3h

Wysyłka i przechowywanie:

The Rat LDHB ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

Inne:

This kit is intended for research use only. Do not use for diagnostics purposes.