ELISA kit for Rat PCK1 (Phosphoenolpyruvate Carboxykinase 1, Soluble)
Gentaur
Mogą Cię zainteresować
A sandwich ELISA kit for detection of Phosphoenolpyruvate Carboxykinase 1, Soluble from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Dodatkowe informacje:
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
- 10nm. The concentration of Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Specyfikacja/Zawartość:
- Species reactivity: Rat
- Sensitivity: 2.66ng/mL
- Detection range: 6.25-100ng/mL
- Standard solution concentration: 100ng/mL
- ELISA type: Sandwich
- Assay length: 3h
Wysyłka i przechowywanie:
The Rat PCK1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
Inne:
This kit is intended for research use only. Do not use for diagnostics purposes.
0 opinie o ELISA kit for Rat PCK1 (Phosphoenolpyruvate Carboxykinase 1, Soluble)