ELISA kit for Mouse FGF4 (Fibroblast Growth Factor 4)

A sandwich ELISA kit for detection of Fibroblast Growth Factor 4 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Dodatkowe informacje:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 4 (FGF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 4 (FGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 4 (FGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
• 10nm. The concentration of Fibroblast Growth Factor 4 (FGF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Specyfikacja/Zawartość:

• Species reactivity: Mouse
• Sensitivity: 3.0pg/mL
• Detection range: 7.8-500pg/mL
• Standard solution concentration: 500pg/mL
• ELISA type: Sandwich
• Assay length: 3h

Wysyłka i przechowywanie:

The Mouse FGF4 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

Inne:

This kit is intended for research use only. Do not use for diagnostics purposes.