ELISA kit for Human VCAM1 (Vascular Cell Adhesion Molecule 1)

A sandwich ELISA kit for detection of Vascular Cell Adhesion Molecule 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Dodatkowe informacje:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
• 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Specyfikacja/Zawartość:

• Species reactivity: Human
• Sensitivity: 0.29ng/mL
• Detection range: 0.78-50ng/mL
• Standard solution concentration: 50ng/mL
• ELISA type: Sandwich
• Assay length: 3h

Wysyłka i przechowywanie:

The Human VCAM1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

Inne:

This kit is intended for research use only. Do not use for diagnostics purposes.