Glutamate is a non-essential amino acid that serves as an important neurotransmitter in the mammalian brain and has a key role in cellular metabolism. Excess glutamate levels in the brain can cause cell injury and death, leading to neurological diseases. Our Glutamate Assay Kit is a quantitative, fluorometric assay that uses glutamate specific enzymes to generate hydrogen peroxide. An ADHP probe is oxidized by hydrogen peroxide to generate fluorescent Resorufin, which correlates to the level of glutamate in the sample. Glutamate levels in an unknown sample are calculated based on a glutamate standard curve.
- Detection method:
Wysyłka i przechowywanie:
- Shipping temperature: Blue Ice
- Storage temperature: Upon receipt, store the 10X Assay Buffer at 4ºC. Aliquot and store all other components at -20ºC. Avoid multiple freeze/thaw cycles. ADHP is light sensitive and must be stored accordingly.