SOD Activity Kit
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Enzyme Activity Colorimetric assay used for quantitative measuring SOD activity in a variety of samples in Serum, Heparin Plasma, EDTA Plasma, Cell Lysates, Tissue, Erythrocytes, Whole Blood, Red Blood Cells samples from all species
Dodatkowe informacje:
- Short-lived and highly reactive oxygen species (ROS) such as O2·- (superoxide), ·OH (hydroxyl radical), and H2O2 (hydrogen peroxide) are continuously generated in vivo. In the resting state, the balance between antioxidants and oxidants is sufficient to prevent the disruption of normal physiologic functions
- however, either increases in oxidants or decreases in antioxidants can disrupt this balance giving rise to elevated levels of reactive oxygen species (ROS) (1,2).The cellular levels of ROS are controlled by antioxidant enzymes and small molecule antioxidants. The major antioxidant enzymes, superoxide dismutases (SODs), including copper-zinc superoxide dismutase (Cu/ZnSOD, SOD1), manganese superoxide dismutase (MnSOD, SOD2) and extracellular superoxide dismutase (EC-SOD, SOD3), all play critical roles in scavenging O2·-. Decreased SOD activity results in elevated level of superoxide which in turn leads to decreased NO but increased peroxynitrite concentrations. The major intracellular SOD is a 32-kD copper and zinc containing homodimer (Cu/Zn SOD). The mitochondrial SOD (MnSOD) is a manganese-containing 93-kD homotetramer that is synthesized in the cytoplasm and translocated to the inner matrix of mitochondria. EC-SOD is the primary extracellular SOD enzyme and is highly expressed in many organs. Increased SOD activity levels are seen in Downs Syndrome (3) while decreased activity is seen in diabetes, Alzheimer's disease, rheumatoid arthritis, Parkinson's disease, uremic anemia, atherosclerosis, some cancers, and thyroid dysfunction (3-8).
Specyfikacja/Zawartość:
- Assay type: Direct Enzyme Activity Assay
- Species reactivity: Species Independent
- Sample types: Serum, Heparin Plasma, EDTA Plasma, Cell Lysates, Tissue, Erythrocytes, Whole Blood, Red Blood Cells
- Sensitivity: 0.044 U/ml
- Assay range: 0.0625 - 4 U/ml
- Incubation t
- Number of samples per kit: 89 samples in duplicate
- Method of detection: Colorimetric Assay
Wysyłka i przechowywanie:
The kit is transported on ice packs (+4ºC). Upon receipt store at 4ºC. Non-hazardous item according to both ADR and UN regulations.
Inne:
For research use only. Not to be used for human or animal treatment or diagnostics.
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