Glutathione S-Transferase Activity Kit

Enzyme Activity Fluorometric assay used for quantitative measuring the activity of glutathione S-transferase in Urine, Serum, EDTA Plasma, Heparin Plasma, Cell Lysates samples from Human, Mollusk, Fish

Dodatkowe informacje:

The Glutathione S-Transferase (GST) family of isozymes function to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione (1). Human GSTs are encoded by 5 gene families, expressing in almost all tissues as four cytosolic and one microsomal forms. Dividing the family by isoelectric points, the basic alpha (pI 8?11), the neutral mu (pI 5?7) and acidic pi (pH<5) classes are populated by additional subclasses, each isozyme displaying differential specificity for given electrophilic molecules (2). Given its pivotal role in ameliorating oxidative stress/damage, GST activity has been repeatedly investigated as a biomarker for arthritis, asthma, COPD, and multiple forms of cancer, as well as an environmental marker (3-7). Examination of GST isoforms and activity in human cancers, tumors and tumor cell lines has revealed the predominance of the acidic pi class. Furthermore, this activity is thought to substantially contribute to the innate or acquired resistance of specific neoplasms to anticancer therapy (8,9).

Specyfikacja/Zawartość:

• Assay type: Direct Enzyme Activity Assay
• Species reactivity: Human, Mollusk, Fish, Toadfish (Batrachoididae), Oyster
• Sample types: Urine, Serum, EDTA Plasma, Heparin Plasma, Cell Lysates
• Sensitivity: 2.70 mU/ml
• Assay range: 7.81 - 500 mU/ml
• Incubation t 30 Minutes
• Number of samples per kit: 40 samples in duplicate
• Method of detection: Fluorometric Assay

Wysyłka i przechowywanie:

The kit is transported on ice packs (+4ºC). Upon receipt store at 4ºC. Non-hazardous item according to both ADR and UN regulations.

Inne:

For research use only. Not to be used for human or animal treatment or diagnostics.