ELISA kit for 8-OHdG (8-Hydroxydeoxyguanosine)
Gentaur
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A competitive ELISA kit for quantitative measurement of 8-OHdG (8-Hydroxydeoxyguanosine) in samples from Serum, Plasma, Cell supernatant
Dodatkowe informacje:
Gentaur's 8-OHdG ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
Specyfikacja/Zawartość:
- Species reactivity: Universal
- Antigen: 8-OHdG
- Assay type: Competitive ELISA
- Sensitivity: 0.94ng/mL
- Detections range: from: 1.56 to: 100ng/mL
- Validated sample types: Serum, Plasma, Cell supernatant
Wysyłka i przechowywanie:
The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.
Inne:
This Universal 8-OHdG ELISA kit is intended for research use only. Do not use for human or animal diagnostics.
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